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NSF Grant Brings Powerful New Microscope to Campus

May 9, 2017

Subramaniam (right), Ghezzehei (center) and Ghosh (left) pose with the new Zeiss LSM 880 confocal microscope.UC Merced added a powerful new tool to the campus’s Imaging and Microscopy Facility. The newly acquired Zeiss LSM 880 is a state-of-the art laser scanning confocal microscope that represents a huge leap forward for the university’s imaging capabilities.

Within UC Merced, this is by far the most advanced, state-of-the-art light microscope,” School of Engineering Professor Anand Subramaniam said.

Subramaniam led the three-person team — which included School of Natural Science professors Sayantani Ghosh and Teamrat Ghezzehei — that brought the microscope to UC Merced. But getting there wasn’t easy.

First, they had to convince the Office of Research and Economic Development (ORED) that the new confocal was among the university’s top research priorities. Subramaniam’s was one of many proposals ORED was considering. But, according to Vice Chancellor for Research Sam Traina, “This was a pretty easy choice.”

The proposal written by Professor Subramaniam and his colleagues was clearly identified as the best of the group by a panel of faculty peers at the university,” Traina said.

With ORED’s endorsement, Subramanian and company next had to convince the National Science Foundation that UC Merced was deserving of a Major Research Instrumentation (MRI) award.

The grant was awarded last September.

So how much does a top-of-the-line confocal microscope cost? A lot, it turns out. The NSF MRI grant covered $311,815, while the School of Natural Sciences, the School of Engineering and ORED covered the remaining $153,228. That’s $465,043 for one microscope.

And according to Subramaniam and Ghosh, it’s worth every penny.

Previously, UC Merced researchers seeking access to comparable equipment had to travel to microscopy facilities housed at Stanford or UC Berkeley. More than just inconvenient, in some cases it was impossible. Some samples are too sensitive to travel; others might survive the journey only to yield data unfit for publication.

Bringing the Zeiss LSM 880 to UC Merced solved this. With the same state-of-the-art equipment on campus, researchers can pursue avenues of inquiry they might otherwise have avoided.

But what makes the microscope such a valuable research tool? The team points to its versatility, power and speed.

It’s important to have multimodal imaging capabilities,” Subramaniam explained. “Most confocals are optimized for biological science research — think squishy, wet things — or materials sciences, with hard things like glass and ceramics. We needed something that could do both”

The confocal has six lasers, allowing for multicolor imaging. It also has the latest Airyscan technology, a feature that minimizes signal loss and brings resolution down to 110 nanometers (for perspective, a human hair is about 100,000 nanometers thick), allowing the microscope to discern details other confocals cannot.

The microscope is also fast. Scientists use confocals because they can capture still images in very quick succession, essentially producing a movie of microscopic processes in action. Typically, there’s a trade-off between speed and resolution. The more seamless the movie, the blurrier the image. This microscope mitigates that problem, providing high-speed, high-resolution dynamic imaging.

For Subramaniam, this feature is key. His lab develops engineering approaches for synthesizing artificial cells. Understanding the dynamic processes involved in the earliest stages of self-assembly of molecules is crucial. How do a bunch of lipids, essentially individual fat molecules, come together to form structures that resemble hollow cells? This microscope will help him and his students figure it out.

For Ghosh, whose lab is developing high-efficiency photovoltaic devices, she and her students need to know how a device’s microstructure affects its performance. To do this, Ghosh needs two kinds of microscopes. They already had one. Now they have the other.

The new confocal complements the Zeiss Gemini SEM 500 scanning electron microscope acquired last year. The confocal’s “shuttle and find” feature bridges the two instruments by allowing users to identify sites of interest within a sample and then transfer the sample to the SEM. The SEM will then automatically probe the same sites, which Ghosh thinks is “the coolest capability of this instrument.”

It can correlate an optical image from the confocal microscope to structural data from the scanning electron microscope,” Ghosh explained. “This will give us unique insights into optimizing and improving photovoltaic devices.”

Subramaniam adds, “This bridging capability allows us to further interrogate our samples down to the 1 nanometer scale.”

But Subramaniam and his collaborators aren’t the only ones excited about the new microscope. As Ghosh put it, “A world-class research university must have significant core facilities to support the needs of its researchers.”

This microscope will do just that. At least 20 faculty, along with their graduate students and research staff members, are expected to regularly use the instrument.

The confocal is an instrument that will benefit the research enterprise at UC Merced,” Subramaniam said. “We encourage users who were not in the initial grant to contact us if they wish to incorporate confocal microscopy into their research programs”

Moreover, this microscope makes UC Merced a magnet for top-tier graduate students, postdocs and faculty. It brings the campus’s imaging facilities on par with long-established research institutes and demonstrates that the university is capable of distinguishing itself when pitted against other Ph.D.-granting institutions competing for the same prestigious grants.

It was clear to all of us that acquisition of this instrument would greatly enhance the research and education environment at UC Merced,” Traina said.